Turk J Hematol. 2025; 42(2): 92-99 | DOI: 10.4274/tjh.galenos.2025.2024.0424

miR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Huichao Wu¹, Lin Zhao², Huanyu Guo³, Yingjie Xie⁴, Jianhua Hu⁵, Xinxia Tan^6
1The First People’s Hospital of Jiashan, Department of Emergency, Jiashan, P.R. China
²Dongzhimen Hospital, Beijing University of Chinese Medicine, Department of Hematology and Oncology, Beijing, P.R. China
³Changchun University of Chinese Medicine, Faculty of Clinical Medicine, Changchun, P.R. China
⁴General Hospital of Southern Theater Command of the Chinese PLA, Departments of Cadre Ward, Guangzhou, P.R. China
⁵People’s Hospital of Quzhou, Department of Intensive Care Medicine, Quzhou, P.R. China
⁶Huangshi Central Hospital (Affiliated Hospital of Hubei Polytechnic University), Department of Clinical Laboratory, Huangshi, P.R. China; Huangshi Tumor Molecular Diagnosis and Treatment Key Laboratory, Huangshi, P.R. China

Objective: Acute myeloid leukemia (AML) highly lethal hematological malignancy that is difficult to treat. This study aimed to clarify the molecular mechanisms of miR-379-5p in AML progression.
Materials and Methods: Quantitative real-time polymerase chain reaction was utilized to evaluate miR-379-5p expression levels in AML patients and a control group. A receiver operating characteristic curve was created to assess the clinical predictive value of miR-379-5p in AML, while cell experiments used the CCK-8 assay, flow cytometry, and transwell chambers. Potential target genes of miR-379-5p were predicted by employing online bioinformatics tools, followed by validation using a dual luciferase reporter assay.
Results: miR-379-5p expression was significantly decreased in AML patients and had clinical predictive value for the disease. In AML cell lines, miR-379-5p was downregulated; conversely, the upregulation of miR-379-5p inhibited proliferation, migration, and invasion while promoting apoptosis. Notably, YBX1 was a potential target gene of miR-379-5p and its upregulation reduced the effects of miR-379-5p on AML cell behavior.
Conclusion: miR-379-5p has potential as a biomarker for AML by regulating cell proliferation and apoptosis through the targeting of YBX1.

Keywords: Acute myeloid leukemia, miR-379-5p, YBX1, Cell proliferation, Apoptosis, Diagnostic significance

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miR-379-5p YBX1 Negatif Düzenlemesi Aracılığı ile Akut Miyeloid Lösemi Hücrelerinde Proliferasyonu İnhibe Etti

Huichao Wu¹, Lin Zhao², Huanyu Guo³, Yingjie Xie⁴, Jianhua Hu⁵, Xinxia Tan^6
1The First People’s Hospital of Jiashan, Department of Emergency, Jiashan, P.R. China
²Dongzhimen Hospital, Beijing University of Chinese Medicine, Department of Hematology and Oncology, Beijing, P.R. China
³Changchun University of Chinese Medicine, Faculty of Clinical Medicine, Changchun, P.R. China
⁴General Hospital of Southern Theater Command of the Chinese PLA, Departments of Cadre Ward, Guangzhou, P.R. China
⁵People’s Hospital of Quzhou, Department of Intensive Care Medicine, Quzhou, P.R. China
⁶Huangshi Central Hospital (Affiliated Hospital of Hubei Polytechnic University), Department of Clinical Laboratory, Huangshi, P.R. China; Huangshi Tumor Molecular Diagnosis and Treatment Key Laboratory, Huangshi, P.R. China

Amaç: Akut miyeloid lösemi (AML) tedavisi zor olan ve oldukça ölümcül bir hematolojik malignitedir. Bu çalışmada AML progresyonunda miR- 379-5p nin moleküler mekanizmalarını netleştirmeyi hedefledik.
Gereç ve Yöntem: AML hastaları ve kontrol grup örneklerinde kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak miR- 379-5p ekspresyon düzeyleri değerlendirildi. AML’de klinik prediktif miR-379-5p değerini ortaya çıkarabilmek için alıcı taraflı karakteristik bir eğri oluşturuldu. Hücre deneyleri ise CCK-8, akım sitometri ve transwell migrasyon tetkikini kullandı. miR-379-5p’nin potansiyel hedefleri online biyoinformatik tahmin araçları ile öngörülmüştür, peşinden dual lusiferaz raportör yöntemi ile doğrulanmıştır.
Bulgular: AML hastalarında miR-379-5p ekspresyonu belirgin şekilde azalmıştır ve hastalık için klinik prediktif değeri olduğu görülmüştür. AML hücre dizelerinde miR-379-5p azalmıştır, oysa ki artmış düzeyleri proliferasyonu, migrasyonu ve invazyonu inhibe ederek apopitozu tetikler. Özellikle YBX1 miR-379-5p geni potansiyel hedefidir ve artmış seviyeleri miR-379-5p nin AML hücre davranışındaki etkilerini azaltmaktadır.
Sonuç: miR-379-5p YBX1’i hedef alarak hücre proliferasyonu ve apopitozisin düzenlenmesi üzerinden AML için potansiyel bir biyobelirteç olabilir.

Anahtar Kelimeler: Akut miyeloid lösemi, miR-379-5p, YBX1, Hücre proliferasyonu, Apopitozis, Tanısal önem

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