1,25(OH)2D3 incubation up-Regulates HOX A9 gene in HL-60 cellsHakan Savlı1, Balint Nagy21Kocaeli University Medical Faculty, Department Of Medical Biology, Kocaeli, Turkey
2Semmelweis University Medical Faculty, 1st Department Of Obstetrics And Gynaecology, Budapest, Hungary
There are many genes involved in vitamin D dependent differentiation of acute myeloid leukaemia cell line HL-60 cells. Involvement of HOX genes in leukaemia and differentiation is just beginning to be appreciated. In order to understand the relationship with 1,25(OH)2D3 dependent differentiation of myeloid leukaemia, we studied the expression of HOX A9 gene. HL-60 cells were exposed to 1,25(OH)2D3 for 24 and 72 hours. Gene expression were investigated using quantitative real-time RT-PCR (LightCycler). HOX A9 levels were found upregulated after 1,25(OH)2D3 treatment, compared to housekeeping RPS9 and HPRT genes. Our study is the first attempt to quantification of HOX A9 gene during 1,25(OH)2D3 treatment, using this technology. We suggest that there is a clear relationship between differentiation induction and over-expression of HOX A9 gene in HL-60 cells. Possible correlation of high expression levels of HOX A9 gene in AML pathogenesis remains to be established. Keywords: Gene expression, HOX A9, Vitamin D, RT-PCR.
1,25(OH)2D3 inkübasyonu HL-60 hücrelerinde HOX A9 geninin upregülasyonuna yol açmaktaHakan Savlı1, Balint Nagy21Kocaeli University Medical Faculty, Department Of Medical Biology, Kocaeli, Turkey
2Semmelweis University Medical Faculty, 1st Department Of Obstetrics And Gynaecology, Budapest, Hungary
Akut miyeloid lösemi hücre dizini olan HL-60 hücrelerinde vitamin D’ye bağımlı farklılaşmada rol oynayan çok sayıda gen vardır. Lösemi ve farklılaflmada HOX genlerinin rolü yeni anlaşılmaya başlamıştır. Miyeloid löseminin 1,25(OH)2D3 bağımlı farklılaflmasını anlamak için bu çalıflmada HOX A9 gen ekspresyonuna bakıldı. HL-60 hücreleri 24 ve 72 saat süre ile 1,25(OH)2D3’le muamele edildi. Gen ekspresyonu kantitatif, gerçek zamanlı PCR ile yapıldı. 1,25(OH)2D3 tedavisinden sonra RPS9 ve HPRT genlerine kıyasla HOX A9 gen düzeyleri yüksek bulundu. Bizim çalıflmamız, bu teknoloji kullanılarak 1,25(OH)2D3 tedavisinde HOX A9 genini ölçen ilk çalı şmadır. Sonuç olarak HL-60 hücrelerinde farklılaşma ile HOX A9 geninin overekspresyonu arasında belirgin ilişki vardır. HOX A9 geninin yüksek düzeyleri ile AML patogenezi ilişkisi araştırma konusudur. Anahtar Kelimeler: Gen ekspresyonu, HOX A9, D vitamini, PCR.
Hakan Savlı, Balint Nagy. 1,25(OH)2D3 incubation up-Regulates HOX A9 gene in HL-60 cells. Turk J Hematol. 2003; 20(4): 217-220
Corresponding Author: Hakan Savlı, Türkiye |
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